THE DEVELOPMENT OF IODATE BASED ANTIOXIDANT CAPACITY DETERMINATION METHOD

Abstract

In the last decade, free radicals have attracted great attention, as they are a natural product of many biological reactions.  Accumulation of the free radicals leads to oxidative stress which plays the essential role of the cell ageing leading to serious diseases. But the cell can defend itself by natural compounds called antioxidants. These defender compounds may be naturally occurred in the cell or can be provided by outside supplements. Antioxidant capacity is determined by several determination methods but they are expensive and complicated. This increased the need to develop an easy, reliable and inexpensive method to determine the antioxidant capacity and that is the goal of this study. In this study the capacity of antioxidant will be determined by the reaction between iodate and hydroxyl amine to release the nitrite which will form a pink colour.